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GFP-Miro1 (1-177 fused to 562-618)
(Plasmid #127627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Miro1
  • Species
    H. sapiens (human)
  • Mutation
    1-177 fused to 562-618
  • Entrez Gene
    RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Miro1 (1-177 fused to 562-618) was a gift from Josef Kittler (Addgene plasmid # 127627 ; http://n2t.net/addgene:127627 ; RRID:Addgene_127627)

  • For your References section:

    Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2. Covill-Cooke C, Toncheva VS, Drew J, Birsa N, Lopez-Domenech G, Kittler JT. EMBO Rep. 2020 Feb 5;21(2):e49865. doi: 10.15252/embr.201949865. Epub 2020 Jan 2. 10.15252/embr.201949865 PubMed 31894645
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