myc-AP4 Epsilon (V454X)
(Plasmid
#127624)
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PurposeN-terminal myc-tagged AP4 epsilon subunit (V454X)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Total vector size (bp) 8174
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAP-4 complex subunit epsilon-1
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Alt nameAP4E1
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SpeciesH. sapiens (human)
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MutationPremature stop codon (V454X)
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Entrez GeneAP4E1 (a.k.a. CPSQ4, SPG51, STUT1)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc-AP4 Epsilon (V454X) was a gift from Josef Kittler (Addgene plasmid # 127624 ; http://n2t.net/addgene:127624 ; RRID:Addgene_127624) -
For your References section:
Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome. Ivankovic D, Drew J, Lesept F, White IJ, Lopez Domenech G, Tooze SA, Kittler JT. Autophagy. 2019 May 29:1-17. doi: 10.1080/15548627.2019.1615302. 10.1080/15548627.2019.1615302 PubMed 31142229