GFP-Trak2
(Plasmid
#127622)
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PurposeN-terminal GFP-tagged mouse Trak2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
- Total vector size (bp) 7475
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrak2
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SpeciesM. musculus (mouse)
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Insert Size (bp)2742
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Entrez GeneTrak2 (a.k.a. 2900022D04Rik, 4733401O11Rik, AI480836, AW046271, Als2cr3, CALS-C, GRIF-1, GRIF1, OIP98, mKIAA0549)
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Trak2 was a gift from Josef Kittler (Addgene plasmid # 127622 ; http://n2t.net/addgene:127622 ; RRID:Addgene_127622) -
For your References section:
Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase. Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT. J Biol Chem. 2014 May 23;289(21):14569-82. doi: 10.1074/jbc.M114.563031. Epub 2014 Mar 26. 10.1074/jbc.M114.563031 PubMed 24671417