myc-Miro2 (lacking TM)
(Plasmid
#127614)
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PurposeHuman Miro2 with the transmembrane deleted
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 127614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Total vector size (bp) 6520
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMiro2
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SpeciesH. sapiens (human)
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MutationTransmembrane deletion
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Entrez GeneRHOT2 (a.k.a. ARHT2, C16orf39, MIRO-2, MIRO2, RASL)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc-Miro2 (lacking TM) was a gift from Josef Kittler (Addgene plasmid # 127614 ; http://n2t.net/addgene:127614 ; RRID:Addgene_127614) -
For your References section:
Miro proteins coordinate microtubule- and actin-dependent mitochondrial transport and distribution. Lopez-Domenech G, Covill-Cooke C, Ivankovic D, Halff EF, Sheehan DF, Norkett R, Birsa N, Kittler JT. EMBO J. 2018 Feb 1;37(3):321-336. doi: 10.15252/embj.201696380. Epub 2018 Jan 8. 10.15252/embj.201696380 PubMed 29311115