pRS416-APS3_intron
(Plasmid
#127605)
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Purposelow-copy URA-selectable plasmid, APS3 intron inserted into URA3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
- Backbone size w/o insert (bp) 4778
- Total vector size (bp) 4855
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAPS3 intron
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)77
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416-APS3_intron was a gift from David Bartel (Addgene plasmid # 127605 ; http://n2t.net/addgene:127605 ; RRID:Addgene_127605) -
For your References section:
Excised linear introns regulate growth in yeast. Morgan JT, Fink GR, Bartel DP. Nature. 2019 Jan;565(7741):606-611. doi: 10.1038/s41586-018-0828-1. Epub 2019 Jan 16. 10.1038/s41586-018-0828-1 PubMed 30651636