Skip to main content
Addgene

pRS416-PTEF1-MCP-no_intron
(Plasmid #127597)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS416
  • Backbone size w/o insert (bp) 4803
  • Total vector size (bp) 5877
  • Modifications to backbone
    MCS locus replaced with 3xFLAG-MCP expression cassette
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1xFLAG-MCP expression cassette
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1074

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS416-PTEF1-MCP-no_intron was a gift from David Bartel (Addgene plasmid # 127597 ; http://n2t.net/addgene:127597 ; RRID:Addgene_127597)

  • For your References section:

    Excised linear introns regulate growth in yeast. Morgan JT, Fink GR, Bartel DP. Nature. 2019 Jan;565(7741):606-611. doi: 10.1038/s41586-018-0828-1. Epub 2019 Jan 16. 10.1038/s41586-018-0828-1 PubMed 30651636
Related items: