pBT2-4xUAS:NICD-P2A-mRFP
(Plasmid #127594)

• Purpose: Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.
• Depositing Lab: Longhou Fang
• Publication: Gu et al Science. 2019 Mar 8;363(6431):1085-1088. doi: 10.1126/science.aav1749. Epub 2019 Jan 31.

Backbone

• Vector backbone: pBT2
• Vector type: Zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NICD
• Alt name: Notch intracellular domain
• Alt name: 4xnr UAS:NICD-P2A-mRFP
• Species: D. rerio (zebrafish)
• Entrez Gene: notch1a (a.k.a. de, des, n, n1a, not, wu:fc01e02, wu:fc37a0, wu:fc37a05)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: TTGGTCACCTTCAGCTTGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry