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PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6
- Backbone size w/o insert (bp) 2894
- Total vector size (bp) 4955
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Vector typeSynthetic Biology ; PCR based tagging
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Selectable markersNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePtetO7.1_Flagtag_8Glycine linker
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SpeciesSynthetic
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Insert Size (bp)2061
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Tag
/ Fusion Protein
- Flag tag, 8glycine linker (C terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRP2375 was a gift from Joerg Stelling (Addgene plasmid # 127581 ; http://n2t.net/addgene:127581 ; RRID:Addgene_127581) -
For your References section:
A precisely adjustable, variation-suppressed eukaryotic transcriptional controller to enable genetic discovery. Azizoglu A, Brent R, Rudolf F. Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549. 10.7554/eLife.69549 PubMed 34342575