FRP2350
(Plasmid #127580)

• Purpose: Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene
• Depositing Lab: Joerg Stelling
• Publication: Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.

Backbone

• Vector backbone: pFA6
• Backbone size w/o insert (bp): 3350
• Total vector size (bp): 5411
• Vector type: Synthetic Biology ; PCR based tagging
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PtetO7.1_Flagtag_8Glycine linker
• Species: Synthetic
• Insert Size (bp): 2061
• Tag / Fusion Protein:
  • Flag tag, 8glycine linker (C terminal on insert)

Resource Information

• Supplemental Documents:
  • FRP2350.gb
  • WTC846 Protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.