FRP2374
(Plasmid #127579)

• Purpose: Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker
• Depositing Lab: Joerg Stelling
• Publication: Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549.

Backbone

• Vector backbone: pRG201
• Backbone size w/o insert (bp): 5279
• Total vector size (bp): 10900
• Vector type: Yeast Expression, Synthetic Biology ; Shuttle Vector
• Selectable markers: MET15

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
• Species: Synthetic
• Insert Size (bp): 5621

Resource Information

• Supplemental Documents:
  • frp2374.gb
  • WTC846 Protocol
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.