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Addgene

FRP2370
(Plasmid #127576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRG205MX
  • Backbone size w/o insert (bp) 5108
  • Total vector size (bp) 10729
  • Vector type
    Yeast Expression, Synthetic Biology ; Shuttle Vector
  • Selectable markers
    LEU2 ; MX

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
  • Species
    Synthetic
  • Insert Size (bp)
    5621

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRP2370 was a gift from Joerg Stelling (Addgene plasmid # 127576 ; http://n2t.net/addgene:127576 ; RRID:Addgene_127576)

  • For your References section:

    A precisely adjustable, variation-suppressed eukaryotic transcriptional controller to enable genetic discovery. Azizoglu A, Brent R, Rudolf F. Elife. 2021 Aug 3;10. pii: 69549. doi: 10.7554/eLife.69549. 10.7554/eLife.69549 PubMed 34342575
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