Skip to main content
Addgene

pES134
(Plasmid #127551)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pKD13
  • Backbone manufacturer
    KEIO collection material
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 3099
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    The insert is stable and the plasmid can be selected on sole ampicillin
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    F3-flanked spectinomycin resistance cassette
  • Alt name
    F3-J23116-B0034m-aadA-F3
  • Species
    Synthetic
  • Insert Size (bp)
    1000

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer cctcgctttgtaacggagtagag
  • 3′ sequencing primer gacggatggcctttttgcgtggc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.17.576040 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pES134 was a gift from Emmanuele Severi & Gavin Thomas (Addgene plasmid # 127551 ; http://n2t.net/addgene:127551 ; RRID:Addgene_127551)

  • For your References section:

    Characterisation of anhydro-sialic acid transporters from mucosa-associated bacteria. Wu Y, Bell A, Thomas GH, Bolam DN, Sargent F, Juge N, Palmer T, Severi E. Microbiology (Reading). 2024 Mar;170(3). doi: 10.1099/mic.0.001448. 10.1099/mic.0.001448 PubMed 38488830
Related items:
Commonly requested with: