INCTbiosyn-pAct-AtINTBxb1
(Plasmid #127536)

• Purpose: Plasmid encodes A. thaliana codon optimized Integrase Bxb1.
• Depositing Lab: Martin Bonamino
• Publication: Gomide et al Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.

Backbone

• Vector backbone: pBluescript II SK(-)
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
• Alt name: integrase Bxb1 Expression vector
• Insert Size (bp): 3197

Cloning Information

• Cloning method: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original Bxb1 coding sequence (before A. thaliana codon optimization) was obtained from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529324.1).