INCTbiosyn-pUB-HspINTphiC31
(Plasmid #127518)

• Purpose: Plasmid encodes H. sapiens codon optimized Integrase phiC31.
• Depositing Lab: Martin Bonamino
• Publication: Gomide et al Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.

Backbone

• Vector backbone: pBluescript II SK(-)
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
• Alt name: integrase phiC31 Expression vector
• Insert Size (bp): 3606

Cloning Information

• Cloning method: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The UbC promoter and _-globin poly(A) signal sequences are derived from pUB-GFP plasmid (Addgene #11155).