INCTbiosyn-pUB-HspINT7
(Plasmid #127515)

• Purpose: Plasmid encodes H. sapiens codon optimized Integrase 7.
• Depositing Lab: Martin Bonamino
• Publication: Gomide et al Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.

Backbone

• Vector backbone: pUB-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Integrase 7 coding sequence codon optimized for H. sapiens expression.
• Alt name: integrase 7 Expression vector
• Insert Size (bp): 1389
• Mutation: In 2126 position an G mutated for an T changed the amino acid from an Glycine for an Cysteine; and in 2526 position an A mutated for an G changed an Tyrosine for an Cysteine.

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • INCTbiosyn-pUB-HspINT7
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The integrase 7 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). The related two SNPs mutations were due to the synthesis building process. The resulted gene coding sequence was the only one possible to be obtained by the synthesis company.