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Addgene

INCTbiosyn-pUB-HspINT5
(Plasmid #127514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUB-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrase 5 coding sequence codon optimized for H. sapiens expression.
  • Alt name
    integrase 5 Expression vector
  • Insert Size (bp)
    1659

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The integrase 5 coding sequence was synthesized and cloned into pUB-GFP plasmid (Addgene #11155) replacing the EGFP coding sequence. The original integrase coding sequence (before H. sapiens codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    INCTbiosyn-pUB-HspINT5 was a gift from Martin Bonamino (Addgene plasmid # 127514 ; http://n2t.net/addgene:127514 ; RRID:Addgene_127514)

  • For your References section:

    Genetic switches designed for eukaryotic cells and controlled by serine integrases. Gomide MS, Sales TT, Barros LRC, Limia CG, de Oliveira MA, Florentino LH, Barros LMG, Robledo ML, Jose GPC, Almeida MSM, Lima RN, Rehen SK, Lacorte C, Melo EO, Murad AM, Bonamino MH, Coelho CM, Rech E. Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8. 10.1038/s42003-020-0971-8 PubMed 32444777
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