INCTbiosyn-pEF-GFP(rc)2
(Plasmid #127504)

• Purpose: Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.
• Depositing Lab: Martin Bonamino
• Publication: Gomide et al Commun Biol. 2020 May 22;3(1):255. doi: 10.1038/s42003-020-0971-8.

Backbone

• Vector backbone: pEF-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
• Alt name: Switch vector INT 2
• Species: H. sapiens (human)
• Insert Size (bp): 842

Cloning Information

• Cloning method: Unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The EGFP coding sequence in a reverse complement orientation flanked by attB and attP integrase 2 attachment sites were cloned into pEF-GFP plasmid (Addgene #11154) replacing the original EGFP forward coding sequence. The attB/P integrase 2 attachment sites sequences were obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).