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pcDNA3.1/Ndi-1/myc-His
(Plasmid #127503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/myc-His(-)A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ndi-1
  • Alt name
    NADH-ubiquinone reductase (H(+)-translocating) NDI1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1539
  • Entrez Gene
    NDI1 (a.k.a. YML120C)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer tgaagaaaCTAAAGCTAGCTATGCTATCGAAGAATTTGTATAGTAA
  • 3′ sequencing primer CTACCAAGGTAATgcggccgcTAATCCTTTAAAAAAGTCTCTTTTGAAAA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/Ndi-1/myc-His was a gift from Shashi Jain & Dieter Wolf (Addgene plasmid # 127503 ; http://n2t.net/addgene:127503 ; RRID:Addgene_127503)

  • For your References section:

    Metabolic targeting of cancer by a ubiquinone uncompetitive inhibitor of mitochondrial complex I. Jain S, Hu C, Kluza J, Ke W, Tian G, Giurgiu M, Bleilevens A, Campos AR, Charbono A, Stickeler E, Maurer J, Holinski-Feder E, Vaisburg A, Bureik M, Luo G, Marchetti P, Cheng Y, Wolf DA. Cell Chem Biol. 2021 Nov 23. pii: S2451-9456(21)00479-7. doi: 10.1016/j.chembiol.2021.11.002. 10.1016/j.chembiol.2021.11.002 PubMed 34852219
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