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Addgene

pZF-BBB
(Plasmid #127470)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127470 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a
  • Backbone size w/o insert (bp) 5344
  • Total vector size (bp) 5607
  • Vector type
    cell-free transcription–translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZF-BBB synthetic transcription factor
  • Insert Size (bp)
    267
  • Promoter T7

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ATGCGTCCGGCGTAG
  • 3′ sequencing primer AACCCCTCAAGACCCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Integrating Gene Synthesis and Microfluidic Protein Analysis for Rapid Protein Engineering. NAR. 2016 April; 44(7): e68. doi:10.1093/nar/gkv1497.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZF-BBB was a gift from Sebastian Maerkl (Addgene plasmid # 127470 ; http://n2t.net/addgene:127470 ; RRID:Addgene_127470)

  • For your References section:

    Cell-free gene-regulatory network engineering with synthetic transcription factors. Swank Z, Laohakunakorn N, Maerkl SJ. Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):5892-5901. doi: 10.1073/pnas.1816591116. Epub 2019 Mar 8. 10.1073/pnas.1816591116 PubMed 30850530
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