pBORF-AD
(Plasmid
#127462)
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Purpose(Empty Backbone) ORF filtering plasmid (RNAp associated plasmid for pAVA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127462 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK(+)
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Backbone manufacturerStratagene #212205
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Modifications to backboneReplaced AmpR with KanR then inserted b-lactamase leader sequence and AmpR in MCS
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Vector typeBacterial Expression
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBORF-AD was a gift from Joel Malek (Addgene plasmid # 127462 ; http://n2t.net/addgene:127462 ; RRID:Addgene_127462) -
For your References section:
High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq). Andrews SS, Schaefer-Ramadan S, Al-Thani NM, Ahmed I, Mohamoud YA, Malek JA. J Biol Chem. 2019 Jul 26;294(30):11549-11558. doi: 10.1074/jbc.RA119.008792. Epub 2019 Jun 10. 10.1074/jbc.RA119.008792 PubMed 31182485