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Flag-MCPIP1 (1-599)
(Plasmid #127461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV-MAT-FLAG-1
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6479
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCPIP1
  • Alt name
    ZC3H12A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1797
  • GenBank ID
    NM_001323550
  • Entrez Gene
    ZC3H12A (a.k.a. MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer No
  • 3′ sequencing primer No
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-MCPIP1 (1-599) was a gift from Mingui Fu (Addgene plasmid # 127461 ; http://n2t.net/addgene:127461 ; RRID:Addgene_127461)

  • For your References section:

    MCP-induced protein 1 deubiquitinates TRAF proteins and negatively regulates JNK and NF-kappaB signaling. Liang J, Saad Y, Lei T, Wang J, Qi D, Yang Q, Kolattukudy PE, Fu M. J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. Epub 2010 Nov 29. 10.1084/jem.20092641 PubMed 21115689
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