EGFP-MCPIP1
(Plasmid #127460)

• Purpose: Expressing EGFP-MCPIP1 fusion protein in mammalian cells
• Depositing Lab: Mingui Fu
• Publication: Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73. doi: 10.1084/jem.20092641. Epub 2010 Nov 29.

Backbone

• Vector backbone: pEGFP/N1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6479
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCPIP1
• Alt name: ZC3H12A
• Species: H. sapiens (human)
• Insert Size (bp): 1797
• GenBank ID: NM_001323550
• Entrez Gene: ZC3H12A (a.k.a. MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ECoRI (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry