Skip to main content
Addgene

pTOX12
(Plasmid #127457)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDS132
  • Total vector size (bp) 5878
  • Modifications to backbone
    Extensive - see paper for details
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    NEEDS 2% GLUCOSE
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tse2 toxin
  • Promoter pRha

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tcatctttccctggttgcca
  • 3′ sequencing primer tcaaacatgagaattcgcgga
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Swaine Chen lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTOX12 was a gift from Matthew Waldor (Addgene plasmid # 127457 ; http://n2t.net/addgene:127457 ; RRID:Addgene_127457)

  • For your References section:

    A New Suite of Allelic Exchange Vectors for the Scarless Modification of Proteobacterial Genomes. Lazarus JE, Warr AR, Kuehl CJ, Giorgio RT, Davis BM, Waldor MK. Appl Environ Microbiol. 2019 Jun 14. pii: AEM.00990-19. doi: 10.1128/AEM.00990-19. 10.1128/AEM.00990-19 PubMed 31201277
Related items:
Commonly requested with: