pH2B-miRFP670nano
(Plasmid #127438)

• Purpose: Histone 2B labeling with near-infrared fluorescent protein miRFP670nano
• Depositing Lab: Vladislav Verkhusha
• Publication: Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-miRFP670nano
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 831
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP670nano (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pH2B-miRFP670nano
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry