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PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepN1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLAMP1-miRFP670nano
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)1716
- Promoter CMV
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Tag
/ Fusion Protein
- miRFP670nano (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLAMP1-miRFP670nano was a gift from Vladislav Verkhusha (Addgene plasmid # 127435 ; http://n2t.net/addgene:127435 ; RRID:Addgene_127435) -
For your References section:
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV. Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8. 10.1038/s41467-018-08050-8 PubMed 30655515
