pEB3-miRFP670nano
(Plasmid #127433)

• Purpose: EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano
• Depositing Lab: Vladislav Verkhusha
• Publication: Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.

Backbone

• Vector backbone: pcDNA
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EB3-miRFP670nano
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1299
• Entrez Gene: MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP670nano (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pEB3-miRFP670nano

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry