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Addgene

pEVOL-pylT-N346A/C348A
(Plasmid #127411)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEVOL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
  • Alt name
    PylRS/Pyl-tRNA
  • Species
    Methanosarcina mazei
  • Mutation
    N346A-C348A
  • Promoter araBAD

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVOL-pylT-N346A/C348A was a gift from Wenshe Liu (Addgene plasmid # 127411 ; http://n2t.net/addgene:127411 ; RRID:Addgene_127411)

  • For your References section:

    A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum. Wang YS, Fang X, Wallace AL, Wu B, Liu WR. J Am Chem Soc. 2012 Feb 15;134(6):2950-3. doi: 10.1021/ja211972x. Epub 2012 Feb 6. 10.1021/ja211972x PubMed 22289053
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