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pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
(Plasmid #127392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127392 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Customized Vector
  • Total vector size (bp) 6921
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    calcium/calmodulin-dependent protein kinase II alpha
  • Alt name
    CaMKIIa
  • Alt name
    Camk2a
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1434
  • Mutation
    changed Threonine 286 to Aspartic acid and Threonine 305/306 to Alanine
  • GenBank ID
    NM_012920.1
  • Entrez Gene
    Camk2a (a.k.a. PK2CDD, PKCCD)
  • Promoter pCAG
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A) was a gift from Ryohei Yasuda (Addgene plasmid # 127392 ; http://n2t.net/addgene:127392 ; RRID:Addgene_127392)

  • For your References section:

    Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in single dendritic spines. Chang JY, Nakahata Y, Hayano Y, Yasuda R. Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z. 10.1038/s41467-019-10694-z PubMed 31239443
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