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PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustomized Vector
- Total vector size (bp) 6921
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecalcium/calmodulin-dependent protein kinase II alpha
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Alt nameCaMKIIa
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Alt nameCamk2a
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1434
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GenBank IDNM_012920.1
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Entrez GeneCamk2a (a.k.a. PK2CDD, PKCCD)
- Promoter pCAG
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCCTCTGCTAACCATGTTC, AGAGCAGCAGGCATGGTTT (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-mEGFP-CaMKIIa was a gift from Ryohei Yasuda (Addgene plasmid # 127389 ; http://n2t.net/addgene:127389 ; RRID:Addgene_127389) -
For your References section:
Mechanisms of Ca(2+)/calmodulin-dependent kinase II activation in single dendritic spines. Chang JY, Nakahata Y, Hayano Y, Yasuda R. Nat Commun. 2019 Jun 25;10(1):2784. doi: 10.1038/s41467-019-10694-z. 10.1038/s41467-019-10694-z PubMed 31239443