UAS-u(S)Cas9
(Plasmid
#127383)
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PurposeExpresses Cas9 at high level
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUASTattB
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Backbone manufacturerJohannes Bischof and Konrad Basler
- Total vector size (bp) 12400
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameuORF(S)-Cas9
- Promoter UAST
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AATCAACTGCAACTACTG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UAS-u(S)Cas9 was a gift from Michael Boutros (Addgene plasmid # 127383 ; http://n2t.net/addgene:127383 ; RRID:Addgene_127383) -
For your References section:
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila. Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdorffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlovic B, Radecke K, Schmitt L, Voos L, Valentini E, Boutros M. Elife. 2020 Feb 13;9. pii: 53865. doi: 10.7554/eLife.53865. 10.7554/eLife.53865 PubMed 32053108
Map uploaded by the depositor.
