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Addgene

pMCRW2
(Plasmid #127380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 3731
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4 repeats of 80 bp DNA template sequence
  • Species
    Synthetic
  • Insert Size (bp)
    334
  • Promoter lac promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCRW2 was a gift from Richard C. Willson (Addgene plasmid # 127380 ; http://n2t.net/addgene:127380 ; RRID:Addgene_127380)

  • For your References section:

    Neutral DNA-avidin nanoparticles as ultrasensitive reporters in immuno-PCR. Chavan D, Chen H, Crum M, Vu B, Safari M, Smith M, Vekilov P, Conrad JC, Kourentzi K, Willson RC. Analyst. 2020 Jul 21;145(14):4942-4949. doi: 10.1039/d0an00134a. Epub 2020 Jun 5. 10.1039/d0an00134a PubMed 32500871
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