pENTR_L1-YFP-Turbo-NES-L2
(Plasmid #127349)

• Purpose: gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins
• Depositing Lab: Dominique Bergmann
• Publication: Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864.

Backbone

• Vector backbone: pENTR
• Total vector size (bp): 4397
• Vector type: gateway entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TurboID (BirA mutant)
• Species: BirA from E. coli, codon optimized for human and mutated
• Insert Size (bp): 957
• Mutation: Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
• Promoter: no promoter
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • GS linker (N terminal on insert)
  • V5 (N terminal on insert)
  • NES (C terminal on insert)
  • GS linker (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 fw
• 3′ sequencing primer: M13 rv

Resource Information

• Supplemental Documents:
  • pENTR_L1-YFP-Turbo-NES-L2.gb
• A portion of this plasmid was derived from a plasmid made by: TurboID was obtained from Alice Ting's lab at Stanford University (cloned from V5-hBirA-Turbo-NES_pCDNA3, Addgene catalog #107169).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry