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Addgene

pCAGGs-BFP
(Plasmid #127348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGs
  • Backbone manufacturer
    Allan Gurtan (Phil Sharp Lab)
  • Total vector size (bp) 5447
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Blue Fluorescent Protein
  • Alt name
    BFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter CAGGS Promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTTCGGCTTCTGGCGTGTGACC
  • 3′ sequencing primer CCCATATGTCCTTCCGAGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGs-BFP was a gift from Phil Sharp (Addgene plasmid # 127348 ; http://n2t.net/addgene:127348 ; RRID:Addgene_127348)
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