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Addgene

6NIM
(Plasmid #127294)

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Item Catalog # Description Quantity Price (USD)
Plasmid 127294 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET15-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid # 26092)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PP-BRD18
  • Alt name
    PDB ID: 6NIM, 6NP7
  • Species
    Trypanosoma cruzi
  • Insert Size (bp)
    360
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID:TcCLB.506553.20:MAC0FE-C02:C243124. SGC or PDB link:
http://www.thesgc.org/structures/6NIM/
https://www.thesgc.org/structures/6NP7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6NIM was a gift from Cheryl Arrowsmith (Addgene plasmid # 127294 ; http://n2t.net/addgene:127294 ; RRID:Addgene_127294)
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