6N31
(Plasmid
#127293)
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PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFBOH-MHL
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Backbone manufacturerSGC (Addgene Plasmid #62304)
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Vector typeBaculovirus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWDR12
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Alt name6N31
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SpeciesH. sapiens (human)
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Insert Size (bp)999
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Entrez GeneWDR12 (a.k.a. YTM1)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: WDR12:JMC061-H05:C224099. SGC or PDB link: http://www.thesgc.org/structures/6N31/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6N31 was a gift from Cheryl Arrowsmith (Addgene plasmid # 127293 ; http://n2t.net/addgene:127293 ; RRID:Addgene_127293)