Skip to main content
Addgene

6N31
(Plasmid #127293)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127293 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    SGC (Addgene Plasmid #62304)
  • Vector type
    Baculovirus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR12
  • Alt name
    6N31
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    999
  • Entrez Gene
    WDR12 (a.k.a. YTM1)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: WDR12:JMC061-H05:C224099. SGC or PDB link: http://www.thesgc.org/structures/6N31/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6N31 was a gift from Cheryl Arrowsmith (Addgene plasmid # 127293 ; http://n2t.net/addgene:127293 ; RRID:Addgene_127293)
Related items:
Commonly requested with: