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Addgene

Myc var 1 K396/397 to NQ
(Plasmid #127286)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEZ-M56
  • Backbone manufacturer
    Genecopoeia
  • Backbone size w/o insert (bp) 7450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myc (NM_001177352)
  • Alt name
    CM665
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1320
  • Mutation
    K396 and 397 to N and Q by Q5 mutagenesi
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GCGGTAGGCGTGTACGGT
  • 3′ sequencing primer CGATCTCGAACTCGTGGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

based on published work for human

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc var 1 K396/397 to NQ was a gift from Christine Milcarek (Addgene plasmid # 127286 ; http://n2t.net/addgene:127286 ; RRID:Addgene_127286)
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