Myc var1 K397R
(Plasmid
#127285)
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PurposeExpresses Myc with putative Ub site mutated
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEZ-M56
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Backbone manufacturerGenecopoeia
- Backbone size w/o insert (bp) 7450
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418) ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMyc (NM_001177352)
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Alt nameCM664
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SpeciesM. musculus (mouse)
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Insert Size (bp)1320
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MutationK397R by Q5 mutagenesis
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Entrez GeneMyc (a.k.a. Myc2, Niard, Nird, bHLHe39)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GCGGTAGGCGTGTACGGT
- 3′ sequencing primer CGATCTCGAACTCGTGGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
based on published work for human
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc var1 K397R was a gift from Christine Milcarek (Addgene plasmid # 127285 ; http://n2t.net/addgene:127285 ; RRID:Addgene_127285)