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Addgene

ELL1 CtoA
(Plasmid #127279)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127279 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEZ-Lv225
  • Backbone manufacturer
    Genecopoeia
  • Backbone size w/o insert (bp) 8565
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin ; eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK)
  • Alt name
    CM659
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
  • Entrez Gene
    ELL (a.k.a. C19orf17, ELL1, MEN, PPP1R68)
  • Promoter EF1a
  • Tag / Fusion Protein
    • Twin strep-tag (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GTTTCGTTTTCTGTTCTGC
  • 3′ sequencing primer AGCAGTCCCCAAGTCAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fails to degrade myc as published for human

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ELL1 CtoA was a gift from Christine Milcarek (Addgene plasmid # 127279 ; http://n2t.net/addgene:127279 ; RRID:Addgene_127279)
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