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Addgene

289aa Met133, 138, 186Ileu ELL2
(Plasmid #127272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF4B
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELL2 w Met to ILeus stopped at XbaI
  • Alt name
    CM563
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    Met133, 138, 186 Ileu, cut with XbaI blunt fillin
  • Entrez Gene
    ELL2 (a.k.a. MRCCAT1)
  • Promoter pEF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NH2 ELL2 should only initiate at Met1 compared to Addgene plasmid 127252, which could initiate at multiple Mets.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    289aa Met133, 138, 186Ileu ELL2 was a gift from Christine Milcarek (Addgene plasmid # 127272 ; http://n2t.net/addgene:127272 ; RRID:Addgene_127272)

  • For your References section:

    Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C. Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13. 10.1038/ni.1786 PubMed 19749764
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