Skip to main content
Addgene

Met133ILeu, Met186ILeu ELL2
(Plasmid #127264)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELL2 (Met133ILeu, Met186ILeu)
  • Alt name
    CM542
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    Two Mets (133,186 )to ILeu Quikchange
  • Entrez Gene
    ELL2 (a.k.a. MRCCAT1)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

see Suppl figure 2 for translation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Met133ILeu, Met186ILeu ELL2 was a gift from Christine Milcarek (Addgene plasmid # 127264 ; http://n2t.net/addgene:127264 ; RRID:Addgene_127264)

  • For your References section:

    Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C. Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13. 10.1038/ni.1786 PubMed 19749764
Related items: