Met331Leu ELL2
(Plasmid
#127262)
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PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 3000
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Vector typein vitro translation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameELL2 (Met331 to Ileu)
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Alt nameCM525
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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MutationMet331 to ILeu Quikchange
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Entrez GeneELL2 (a.k.a. MRCCAT1)
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
see Suppl figure 2 for translation
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Met331Leu ELL2 was a gift from Christine Milcarek (Addgene plasmid # 127262 ; http://n2t.net/addgene:127262 ; RRID:Addgene_127262) -
For your References section:
Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C. Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13. 10.1038/ni.1786 PubMed 19749764