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Addgene

frameshift ELL2
(Plasmid #127257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127257 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELL2
  • Alt name
    CM520
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1970
  • Mutation
    XhoI site between the 1st &2nd Met was filled in, resulting in frameshift that generates a 58kDa protein (compared to 80kDa full-length)
  • Entrez Gene
    ELL2 (a.k.a. MRCCAT1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

XhoI filled in, creates new PvuI, frameshifts protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    frameshift ELL2 was a gift from Christine Milcarek (Addgene plasmid # 127257 ; http://n2t.net/addgene:127257 ; RRID:Addgene_127257)

  • For your References section:

    Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C. Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13. 10.1038/ni.1786 PubMed 19749764
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