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Addgene

ELL2mRNA+polyA
(Plasmid #127256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    in vitro translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELL2
  • Alt name
    CM519
  • Alt name
    IMAGE Clone4822236
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1970
  • Mutation
    shortens 3'UTR to 40 bp adds A30 tail
  • Entrez Gene
    ELL2 (a.k.a. MRCCAT1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE Clone 4822236

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HindIIIAGCTTGGA(Ax30)CGCGT/AACCCC. This is the parent plasmid for the ELL2 Met mutants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ELL2mRNA+polyA was a gift from Christine Milcarek (Addgene plasmid # 127256 ; http://n2t.net/addgene:127256 ; RRID:Addgene_127256)

  • For your References section:

    Transcription elongation factor ELL2 directs immunoglobulin secretion in plasma cells by stimulating altered RNA processing. Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C. Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009 Sep 13. 10.1038/ni.1786 PubMed 19749764
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