Skip to main content
Addgene

IgH P+ VA20
(Plasmid #127249)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127249 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCR2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Promoter+V-region of IgH of A20 B cell line
  • Alt name
    CM534
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    828
  • Promoter IgH

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence is show in Figure 2 of the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IgH P+ VA20 was a gift from Christine Milcarek (Addgene plasmid # 127249 ; http://n2t.net/addgene:127249 ; RRID:Addgene_127249)

  • For your References section:

    Increased phosphorylation of the carboxyl-terminal domain of RNA polymerase II and loading of polyadenylation and cotranscriptional factors contribute to regulation of the ig heavy chain mRNA in plasma cells. Shell SA, Martincic K, Tran J, Milcarek C. J Immunol. 2007 Dec 1;179(11):7663-73. PubMed 18025212
Related items: