IgH P+ VA20
(Plasmid
#127249)
-
PurposeIgH V-region
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127249 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCR2.1
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3900
-
Vector typeOther
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePromoter+V-region of IgH of A20 B cell line
-
Alt nameCM534
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)828
- Promoter IgH
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence is show in Figure 2 of the associated publication.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
IgH P+ VA20 was a gift from Christine Milcarek (Addgene plasmid # 127249 ; http://n2t.net/addgene:127249 ; RRID:Addgene_127249) -
For your References section:
Increased phosphorylation of the carboxyl-terminal domain of RNA polymerase II and loading of polyadenylation and cotranscriptional factors contribute to regulation of the ig heavy chain mRNA in plasma cells. Shell SA, Martincic K, Tran J, Milcarek C. J Immunol. 2007 Dec 1;179(11):7663-73. PubMed 18025212
