pCDNA3.1-V5-ORP7
(Plasmid
#127235)
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PurposeEukaryotic expression of ORP7
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127235 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameORP7
- Promoter CMV
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.1-V5-ORP7 was a gift from Kai Johnsson (Addgene plasmid # 127235 ; http://n2t.net/addgene:127235 ; RRID:Addgene_127235) -
For your References section:
Exploiting ligand-protein conjugates to monitor ligand-receptor interactions. Haruki H, Gonzalez MR, Johnsson K. PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 2012 May 31. 10.1371/journal.pone.0037598 PubMed 22701522
