pAN1572
(Plasmid
#127202)
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PurposeDual inducible expression of Venus-degronSwitchA and KeyA-BFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom MoClo Backbone
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Vector typeSynthetic Biology
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAN1572 was a gift from Hana El-Samad (Addgene plasmid # 127202 ; http://n2t.net/addgene:127202 ; RRID:Addgene_127202) -
For your References section:
De novo design of bioactive protein switches. Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, El-Samad H, Baker D. Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 10.1038/s41586-019-1432-8. 10.1038/s41586-019-1432-8 PubMed 31341284