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Addgene

pJ411 FUS 1-163 Y24xF
(Plasmid #127196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RP1B
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS 1-163 Y24xF
  • Alt name
    FUS LC Y24xF
  • Species
    H. sapiens (human)
  • Mutation
    Y24xF: Y6F, Y14F, Y17F, Y25F, Y33F, Y38F, Y41F, Y50F, Y55F, Y58F, Y66F, Y75F, Y81F, Y91F, Y97F, Y100F, Y113F, Y122F, Y130F, Y136F, Y143F, Y149F, Y155F, Y161F
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Tag / Fusion Protein
    • hexahistidine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ411 FUS 1-163 Y24xF was a gift from Nicolas Fawzi (Addgene plasmid # 127196 ; http://n2t.net/addgene:127196 ; RRID:Addgene_127196)

  • For your References section:

    Molecular interactions underlying liquid-liquid phase separation of the FUS low-complexity domain. Murthy AC, Dignon GL, Kan Y, Zerze GH, Parekh SH, Mittal J, Fawzi NL. Nat Struct Mol Biol. 2019 Jul;26(7):637-648. doi: 10.1038/s41594-019-0250-x. Epub 2019 Jul 1. 10.1038/s41594-019-0250-x PubMed 31270472
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