RP1B FUS 1-163
(Plasmid #127192)

• Purpose: Protein expression for affinity purification
• Depositing Lab: Nicolas Fawzi
• Publication: Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-648. doi: 10.1038/s41594-019-0250-x. Epub 2019 Jul 1.

Backbone

• Vector backbone: RP1B
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUS 1-163
• Alt name: FUS LC
• Alt name: FUS LC WT
• Species: H. sapiens (human)
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
• Tag / Fusion Protein:
  • hexahistidine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry