pVB004
(Plasmid
#127188)
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PurposeTunable NOT gate without RiboJ insulator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127188 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAN1201
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Backbone manufacturerAlec Nielsen
- Backbone size w/o insert (bp) 4921
- Total vector size (bp) 6886
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTunable NOT gate and Reporter without RiboJ Insulator
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SpeciesSynthetic; P. fluorescens
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Insert Size (bp)1965
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVB004 was a gift from Thomas Gorochowski (Addgene plasmid # 127188 ; http://n2t.net/addgene:127188 ; RRID:Addgene_127188) -
For your References section:
Tunable genetic devices through simultaneous control of transcription and translation. Bartoli V, Meaker GA, di Bernardo M, Gorochowski TE. Nat Commun. 2020 Apr 29;11(1):2095. doi: 10.1038/s41467-020-15653-7. 10.1038/s41467-020-15653-7 PubMed 32350250