pEBTet-SNAP-HCA_sensor
(Plasmid
#127182)
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PurposeEukaryotic expression of the fusion protein used for sulfa-drugs sensing
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEBTet
- Total vector size (bp) 14409
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
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SpeciesSynthetic
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Insert Size (bp)2991
- Promoter CMV-TetO2
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Tags
/ Fusion Proteins
- StrepTag (N terminal on insert)
- HisTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBTet-SNAP-HCA_sensor was a gift from Kai Johnsson (Addgene plasmid # 127182 ; http://n2t.net/addgene:127182 ; RRID:Addgene_127182) -
For your References section:
Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins. Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T, Johnsson K. ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 10.1021/acssensors.7b00331. Epub 2017 Aug 2. 10.1021/acssensors.7b00331 PubMed 28766337
